AB0031 METABOLIC ADAPTATION OF HUMAN NEUTROPHILS TO GLUCOSE DEPRIVATION IS IMPAIRED WITH INCREASING AGE

Background: Age-related impairment of classical neutrophil functions is well described (Fortin, McDonald et al. 2008). However, experimental evidence for age-related alterations metabolic adaptation towards nutrient deprivation -a feature battlefields- remains elusive. Moreover, differences in may contribute to pathologies such as atherosclerosis, cancer, and autoimmune diseases including rheumatoid arthritis. Objectives: Therefore, we hypothesized that human neutrophils glucose impaired with increasing age. Methods: We isolated peripheral CD15+ from four healthy young donors (mean age: 23.4 ± 2.7) a mean age 58.7 2.4. First, analyzed the survival either stimulated PMA or left untreated subsequently incubated 0 h 6 under varying concentrations (0, 0.5, 1, 5, 10 mM). To address this, used 7-AAD staining flow cytometry. Using Seahorse™ technology, determined basal respiration, ATP-bound maximal spare capacity. Results: show (purity > 95%) survived hours vitro , independent treatment culture medium. With negligible between various used, percentage living cells after was 95% 2.5 without 75% 4.7 stimulation. No were uncovered this respect two groups. technology revealed significant basal, maximal, respiratory Briefly, OCR (pmol/min/cell count) reserve capacity lower elderly compared donors. For instance, concentration 5 mM glucose, respiration (OCR) 17 0.7 22.5 1 donors, while 25 0.8 41 0.6 donor group. Interestingly, these Conclusion: Our data parameters differ older Further experiments are needed understand detail mechanisms effects metabolism on functions. References: [1]Fortin, C. F., P. McDonald, O. Lesur T. Fülöp, Jr. (2008). “Aging neutrophils: there still much do.” Rejuvenation Res 11 (5): 873-882. Disclosure Interests: None declared